A7470

Sigma

 

Anti-c-Myc Agarose Affinity Gel antibody produced in rabbit

affinity isolated antibody

Synonym:Anti-c-Myc

Description

ApplicationAnti-c-Myc conjugated to agarose beads for the immunoprecipitation or immunoaffinity purification of fusion proteins.
Immunogensynthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.
SpecificitySpecificity: N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.
Physical formSuspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Properties

antibody formaffinity isolated antibody
clone(polyclonal)
species reactivityhuman
application(s)immunoprecipitation: suitable
capacity 2 nmol/mL, gel binding capacity (c-myc fusion protein)
Gene Informationhuman ... MYC(4609)
storage temp.2-8°C

Safety

WGK Germany2

Related Products

Related productE6654, EZview™ Red Anti-c-Myc Affinity Gel
 P8170, Protein G−Peroxidase from Streptococcus sp.
 IP0020, Anti-c-Myc Immunoprecipitation Kit

References

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 Campbell, A.M., et al., The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane. J. Biol. Chem. 267, 9321-9325, (1992)
 Hu, Q., et al., Ras-dependent induction of cellular responses by constitutively active phosphatidylinositol-3 kinase. Science 268, 100-102, (1995)
 Kobayashi, H., et al., Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits. Mol. Biol. Cell 11, 1279-1294, (1992)
 Gosney, J.R., et al., c-myc oncoprotein in bronchial carcinoma: expression in all major morphological types. Anticancer Res. 10, 623-628, (1990)
 Karayiannis, M., et al., Evaluation of the ras and myc oncoproteins in benign gastric lesions. Anticancer Res. 10, 1127-1133, (1990)
 Tiniakos, D., et al., Expression of ras and myc oncogenes in human hepatocellular carcinoma and non-neoplastic liver tissues. Anticancer Res. 9, 715-721, (1989)
 Androniki, T., et al., Implication of the ras and myc oncoproteins in the pathogenesis of mycosis fungoides. Anticancer Res. 11, 1433-1438, (1991)
 Williams, A.R.W., et al., Immunohistochemical demonstration of altered intracellular localization of the C-Myc oncogene product in human colorectal neoplasms. J. Pathol. 160, 287-293, (1990)
 Royds, J.A., et al., Cellular localisation of c-myc product in human colorectal epithelial neoplasia. J. Pathol. 166, 225-233, (1992)
 Robertson, D., et al., Ultrastructural localization of ras-related proteins using epitope-tagged plasmids. J. Histochem. Cytochem. 43, 471-480, (1995)
 Kleymann, G., et al., Use of antibody fragments (Fv) in immunocytochemistry. J. Histochem. Cytochem. 43, 607-614, (1995)